|
KMID : 1041220000420010048
|
|
Journal of Sericultural and Entomological Science
2000 Volume.42 No. 1 p.48 ~ p.57
|
|
|
Enzymatic hydrolysis of insoluble silk sericin by Alcalase
|
|
Jung Hye-Young
Bae Do-Gyu
|
|
|
Abstract
|
|
|
|
This study was undertaken to figure out the effects of hydrolysis conditions on the solubility of insoluble sericin, molecular weight distribution and thermal characteristics of hydrolysates in enzymatic hydrolysis by Alcalase 2.5 L. It was indicated that the optimum treatment temperature and pH for the insolucle sericin were 50°C and 1). respectively. When the insoluble sericin was hydrolyzed with a vatious treatment conditions. the solubility of all hydrolysates were represented above 85% at given conditions. As the enzyme concentration increased. As the treatment time increased, the solubility increased roughly, but the solubility was also increased. It was showed in the molecular weihg distribution of hydrolysates treated various enzyme concentrations and treatment times that when enzyme concentration were 0.5, 2, 3%, the peaks of the distribution curve were shifted to left side which meant low molecular weight and was distributed much quantity with treatment times. When enzyme concentration was 1% and treatment time was below 4hr., the peak was shifted to left side, but treatment time was 6hr. the peak were shifted to right side. When enzyme concentration was 5% and treatment time was below 2hr.. the peaks were shifted to right side. but treatment time was above 4hr. the peak was shifted to left side. The number-average molecular weight were distributed from 300 to 800 and thise were decreased when treatment time was up to 4hr., but increased a little when treatment time was 6hr. It was showed in the DSC curves of hydorolysates depending on treatment times had a tendency to shift to higher temperature. But, when the treatment time was 6hr.. the peak was shifted to lower temperature comparing another hydrolysates.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|